Two glass plates are joined along one edge by a hinge strip of silicone rubber, and the vinyl side spacers 1. Ettan ipgphor 3 equipo medico descargar manual en pdf. Ge healthcare ettan daltsix user manual pdf download manualslib. Ettan ipgphor 3 with ettan ipgphor manifold offers a dedicated platform for running up to 12 firstdimension ief separations with immobiline drystrip gels. Proteome analysis identifies the dpr protein of streptococcus. Dye characteristics cydye dige fluor cy3 saturation dye molecular formula c 37 h 44 n 4 o 6 s formula weight 672. The ettan daltsixsystem uses the same cassettes as the ettan dalttwelve electrophoresis system see fig 3. Pdf proteomic profiling of the epithelialmesenchymal. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis.
Oct 02, 2010 2 aaaaaaaaaaaaaaaaaaaaaa aaaaaaaaaaaaaaaa preface proteomics is the largescale screening of the proteins of a cell, organism or biological fluid, a process which requires stringently controlled steps of. The bosch company makes kitchen and home appliances, and has a line of highend appliances. Are you trying to find electrophoresis available on sale. Ettan daltsix electrophoresis system user manual 80649249 edition ac 7 1 ettan daltsix electrophoresis. Multiphor ii flatbed electrophoresis unit is another alternative. Ettan ipgphor cup loading manifold user manual contains instructions for firstdi mension isoelectric focusing of proteins on ipg strips.
For detailed instructions on installation and usage of this software, refer to the ettan ipgphor 3 control software user manual. Advertisement the 1950s appear to have been a time when t. On the day the auction is set to close, each lot will clearly countdown the time left until closing. Why the next great technology breakthrough shouldnt need a user manual. Breathe easier with our openairways guide to better workouts, less coughing and wheezing, and just maybe a longer life. Ipgphor ipgbox immobiline drystrip gels ipg buffer ettan daltsix ettan dalttwelve dige gel and dige buffer kit typhoon fla 9000 decyder 2d software v7. The protein facility of the iowa state university office of biotechnology is open to faculty and students from the university, other educational institutions, and industry scientists. The small tshaped protrusion fits into a cutout section of the ipgphor bed near the lid hinge fig 29. Ettan ipgphor 3 ief system cytiva, formerly ge healthcare life.
If you own a ge appliance, its important to have an owners manual to ensure proper maintenance and to answer any questions you may have. For questions about willmakers documents and interviews, see also willmaker faqs. If you have one or several of these appliances and need a user manual, there are a few places you may be able to find one online. Are you trying to find electrophoresis or other similiar items. Early rate through december 4 technology is part of a modern fascination wi.
Ge laboratory equipment user manuals download manualslib. Amersham cydye dige fluor labeling kit for scarce samples. The manifold is also compatible with the firstgeneration ettan ipgphor. User manual user manual operating instructions manual. Ief using ettan ipgphor 3 isoelectric focusing system and. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold, ettan ipgphor manifold light, or cer. Description for use with ettan ipgphor 3 isoelectric focusing system specification dimtype instrument user manual unit 0 qualifier. Rehydration was performed overnight at room temperature. Cytiva formerly ge healthcare life sciences polymeric.
Mar 27, 2015 the strips were rehydrated and run in an ettan ipgphor 3 instrument with an adequate length of strip holders ettan ipgphor 3 fixedlength strip holders, ge healthcare. Ettan ipgphor 3 manifold ettan ipgphor 3 manifold is an accessory for firstdimension ief of proteins on ipg strips. Detailed info and photos for this can be found on our website at ettan ipgphor 3 iefisoelectricfocusingprotein2delectroph. Control software enables you to start, stop, and pause ief runs on the ettan ipgphor 3 monitor voltage, current, and volthours of the run and generate a graphical display as it. The facility offers proteinpeptide sequencing, large and small scale peptide synthesis fmoc, matrixassisted laser desorptionionization maldi mass spectrometry, circular dichroism, sdspageblotting, 2d. Run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold, ettan ipgphor manifold light, or ceramic strip holders. The user can create, save, and edit protocols and also pause during a run to edit a protocol in real time.
Ge healtcare life sciences ettan ipgphor 3 isoelectric focusing unit contact. User friendly ettan ipgphor 3 control software is a user friendly interface to the ettan ipgphor. Ge healthcare ettan ipgphor 3 ief system with ceramic manifold and ipg box. Ettan ipgphor 3 ief system from ge healthcare used. Shop a large selection of electrophoresis system accessories products and learn more about cytiva formerly ge healthcare life sciences ettan ipgphor manifold light manifold. Connect only to coolant sources with regulated pressure. Preparation, labelling, and detection of different protein. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold, ettan ipgphor manifold light, or ceramic strip holders. Ettan ipgphor 3 control software ettan ipgphor 3 control software generates professional graphical reports with a large user interface for programming and editing. Change instrument if needed by pressing the correct instrument control button. The seconddimension electrophoresis separates the proteins on the basis of their. Ettan ipgphor 3 ief system cytiva, formerly ge healthcare.
Better workouts, less coughing and wheezing, even a longer life. Ettan ipgphor 3 unit has undergone a complete redesign to provide an even higher level of quality and reproducibility with improved sample handling and pc control. Often filled with jargon, acronyms, and directions that require a ph. Miniprotean ii cell components are not compatible with chlorinated hydrocarbons e.
Ettan ipgphor 3 isoelectric focusing unit 45002623 unit to run first dimension ief ipgphor manifold, complete 450026 for the most stringent applications. As a result, the guide may make assumptions about th. Mar 16, 2020 ief is performed using the ettan ipgphor 3 isoelectric focusing system from ge healthcare life sciences. Ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, speed, reproducibility and high proteinloading capacity. Ettan ipgphor 3 isoelectric focusing unit ge healthcare, cat. Isoelectric focusing electrophoresis system ettan ipgphor 3. The ettan ipgphor cup loading manifold user manual contains instructions for firstdimension isoelectric focusing of proteins on ipg strips.
This manual is also available on the ge healthcare website. A0 0603 ettan ipgphor cup loading manifold ettan ipgphor isoelectric focusing system. Ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimised to deliver high throughput, speed, reproducibility, and high proteinloading capacity. Divided into compartments with a platform in the middle, the horizontal electrophoresis systems completely submerge plated gel platforms in a buffer. Ettan ipgphor 3 control software allows easy programming and viewing of run data. To control multiple units from one computer, an optional junction box is necessary. Ipgphor manifold light 45002621 routine applications.
The software can be used to control up to four ettan ipgphor 3 ief units. Synchronized solutions for better 2d electrophoresis. Proteins extracts 50 g were loaded on ipg strips ph 47 and 69 via a sample cup and subjected to first dimension isoelectric focusing using an ettan ipgphor 3 ge healthcare, uppsala, sweden as follow. Amersham bioscience ettan spot picker with protective hood.
Run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold, ettan ipgphor. The excellent first dimension separation ensures that spots in the second dimension are well separated fig 4. Cytiva formerly ge healthcare life sciences 11002580 catalog no. With neutral narrow range ph intervals 3 7 nl, 47 and 5. Ettan dige applications are described in detail in the ettan dige user manual. Polymeric manifold tray for ettan ipgphor 3 isoelectric focusing unit manufacturer. Typhoon fla 9000 quick start guide baylor university. For detailed instructions on installation and usage of ettan ipgphor control software, and also on computer instrument connection, please refer to the ettan ipgphor software user manual. Amersham pharmacia biotech 3 ipgphor isoelectric focusing system user manual control panel the ipgphor is controlled by a 7key membrane keypad. Shop our great selection, or try a simple search for a precise john deere tractor using the search. Use 2% ph 3 10 ipg buffer in the sample for all ph intervals. Ettan ipgphor 3 control software user manual 11003459 edition ab 15 3. Ipgphor ipgbox immobiline drystrip gels ipg buffer ettan daltsix ettan dalttwelve dalt gel and dalt buffer kit deep purple total protein stain imagequant 401040004000 mini imagemaster 2d platinum ettan spot picker ettan digester sample preparation protein separation blotting antibody probing detection image acquisition image. Cytiva formerly ge healthcare life sciences manifold kit.
Ettan ipgphor 3 isoelectric focusing unit 1 11003364 ipgphor manifold ceramic tray 1 80649857 electrode set 1 80649876 sample cups 120 80649895 ipgphor strip holder cleaning solution 950 ml 80645278. Ettan ipgphor 3 control software user manual 11003459 ettan ipgphor 3 instrument user manual 11003458 ettan ipgphor cup loading manifold user manual 11003460 2d electrophoresis principles and methods 80642960 ettan dalttwelve system user manual 80647653. This system uses immobilized ph gradient ipg gels with a plastic backer. Ettan ipgphor manifold light complete ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold. Page 1 ge healthcare ettan daltsix electrophoresis system user manual page 3 12 psig. Ettan ipgphor 3 control software user manual 11003459 edition ab 7 1introduction 1. Ettan ipgphor manifold comes with a complete set of accessories to run 10 full runs of 12 strips each, including forceps, cleaning brush, ipgphor cleaning solution and a spirit level. Ge ettan ipgphor 3 ief isoelectric focusing protein 2d. The protein facility of the iowa state university office. Twodimensional separation of protein samples sigmaaldrich. Ettan ipgphor isoelectric focusing unit 80641402 typhoon 9400 variable mode imager 63003854 ettan dalt gel 12. Ettan ipgphor 3 isoelectric focusing unit cytiva, formerly. Ettan ipgphor 3 isoelectric focusing system function and description 1 ettan ipgphor 3 instrument user manual 11003458 edition ab control panel the ett an ipgphor 3 is controlled by a 7key membrane keypad.
Ettan ipgphor 3 control software can be used to control up to four ettan ipgphor 3 units at one time, each running a different program. D to understand, software user manuals are sometimes written from the point of view of a developer rather than a user. Ettan ipgphor 3 instrument user manual 11003458 edition ab 9 1. The lots will have a staggered closing time with 2 closing every minute in numerical order. Our site represents a big variety of electrophoresis, displaying listings such as massey ferguson, baler, front loader, zero turn, and many extra. Weve all been thereyou moved to a new home or apartment, and its time to set up electronics and components.
The protein facility of the iowa state university office of. These gels increase reproducibility, loading capacity and are easier to handle. Up to four ettan ipgphor 3 units can be simultaneously controlled by the ettan ipgphor 3 control software, each processing a different set of run parameters. The manifold is designed to handle ief and subsequent equilibration for up to 12 ipg strips. Miniprotean ii electrophoresis cell instruction manual. Ettan ipgphor 3 is optimised for easy handling of cydye labelled proteins and other lightsensitive samples, and provides temperature control critical for reproducibility in 2d dige experiments. Programming from the control panel or in ettan ipgphor 3. Data file 28937442 aa 2d electrophoresis ipgbox and. The ettan ipgphor ii from ge healthcare is the ideal option for obtaining perfectly resolved first dimensions of 2d gels. If any part is missing, please contact your local ge healthcare sales of fice.
Ge healthcare ettan daltsix user manual pdf download. Ge healthcare instrument user manual for ettan ipgphor 3. Use this online manual answers basic questions about using quicken willmaker plus. The manufacturer claims that the system provides reproducibility, reliability, speed, accuracy, high protein loading capacity, and high throughput, and in my experience, the system really stands up to their claim. General electric ge appliances offers consumer home appliances. Ettan ipgphor 3 control software generates professional graphical reports with a large user interface for programming and editing. Ge healthcare ettan ipgphor3 system used tigerfrog scientific.
Get smooth, soft, youngerlooking skin with these skin tips from top dermatologists. Ensure that the manifold is level by placing the round spirit level on the center of the manifold tray after it is placed on the ettan ipgphor 3 unit. Ettan ipgphor 3 ief system from bd biosciences selectscience. The software can be used to control up to four ettan ipgphor 3 ief units simultaneously, each running a different set of run parameters. The improved, easyto use ettan ipgphor 3 system overcomes the most common hurdles of existing technology, to consistently deliver speed, reproducibility. The uninstall ettan ipgphor ii control software program will start. Run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifol. Used ge healthcare ettan ipgphor3 system chemistry analyzer. For creating, editing, and running protocol programs from the ettan ipgphor control panel, please refer to the ettan ipgphor 3 instrument user manual. Except, when you bought them, you didnt think youd need the user manuals after initially setting them up. User manual specifications user manual user manual user manual owners manual user manual instructions for use manual product information sheet. Ettan ipgphor 3 isoelectric focusing system, cytiva vwr.
After first dimension separation, strips were incubated in equilibration. Imagequant las 4010 4000 4000 mini quick start guide. Ge healthcare ettan ipgphor3 system used verified powerup. Tech startups should aspire to foster an intuitive relationship with technology and not get distracted by wiz bang promises. A00603 ettan ipgphor ii isoelectric focusing system. The default installation of the ettan ipgphor ii control software will be on the local c drive within the folder titled amersham biosciences. The kubark manual was written by the cia in the 1960s as a means of standardizing interrogation techniques. User friendly ettan ipgphor 3 control software is a userfriendly interface to the ettan ipgphor. In each compartment, electrodes produce the electric field to cause. If you think an accessory adapters, power cables, probes, and manuals etc.
752 137 133 321 188 1305 1728 683 1379 1591 1662 1594 1768 153 1038 1528 1008 1787 453 1012 1716 1683 722 1493 1674 846